Recombinant:Article Title: Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Saccharomyces cerevisiae Open Biosystems BY4741 and derivatives—Appendix Table S3 Recombinant DNA DNA plasmids Appendix Table S1 Appendix Table S1 Antibodies HRP Goat Anti-Rabbit IgG BD Biosciences 554021 Anti-Dgk1 Han et al, 2008b Han et al, 2008b Oligonucleotides and other sequence-based reagents Oligonucleotides Appendix Table S2 Appendix Table S2 Chemicals, enzymes, and other reagents BODIPY 493/503 Invitrogen D3922 Oleic Acid Sigma Aldrich 05508 Monodansylpentane (MDH) Abcepta SM1000a Water for UHPLC suitable for mass spectrometry (MS) Sigma Aldrich 900682-1L Acetonitrile, MS SupraSolv Sigma Aldrich 1006651000 Methanol, HPLC grade Sigma Aldrich 34860-1L-R Chloroform Sigma Aldrich C2432-1L Isopropanol Optima LC/MS Grade Fisher Scientific 10091304 Ammonium formate Sigma Aldrich 516961-100G SPLASH LpidoMIX Mass Spec Standard Avanti Polar Lipids 330707 Software Image J https://imagej.net/ij/ ImageJ 2.1.0/1.53c © The Author(s) The EMBO Journal 13 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number Imaris https:// imaris.oxinst.com/ Imaris 10.1.0 Prism https:// www.graphpad.com/ Prism 10.2.3 Adobe Photoshop https:// www.adobe.com/ Photoshop 2024 Other
Sequencing:Article Title: Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Saccharomyces cerevisiae Open Biosystems BY4741 and derivatives—Appendix Table S3 Recombinant DNA DNA plasmids Appendix Table S1 Appendix Table S1 Antibodies HRP Goat Anti-Rabbit IgG BD Biosciences 554021 Anti-Dgk1 Han et al, 2008b Han et al, 2008b Oligonucleotides and other sequence-based reagents Oligonucleotides Appendix Table S2 Appendix Table S2 Chemicals, enzymes, and other reagents BODIPY 493/503 Invitrogen D3922 Oleic Acid Sigma Aldrich 05508 Monodansylpentane (MDH) Abcepta SM1000a Water for UHPLC suitable for mass spectrometry (MS) Sigma Aldrich 900682-1L Acetonitrile, MS SupraSolv Sigma Aldrich 1006651000 Methanol, HPLC grade Sigma Aldrich 34860-1L-R Chloroform Sigma Aldrich C2432-1L Isopropanol Optima LC/MS Grade Fisher Scientific 10091304 Ammonium formate Sigma Aldrich 516961-100G SPLASH LpidoMIX Mass Spec Standard Avanti Polar Lipids 330707 Software Image J https://imagej.net/ij/ ImageJ 2.1.0/1.53c © The Author(s) The EMBO Journal 13 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number Imaris https:// imaris.oxinst.com/ Imaris 10.1.0 Prism https:// www.graphpad.com/ Prism 10.2.3 Adobe Photoshop https:// www.adobe.com/ Photoshop 2024 Other
Mass Spectrometry:Article Title: Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Saccharomyces cerevisiae Open Biosystems BY4741 and derivatives—Appendix Table S3 Recombinant DNA DNA plasmids Appendix Table S1 Appendix Table S1 Antibodies HRP Goat Anti-Rabbit IgG BD Biosciences 554021 Anti-Dgk1 Han et al, 2008b Han et al, 2008b Oligonucleotides and other sequence-based reagents Oligonucleotides Appendix Table S2 Appendix Table S2 Chemicals, enzymes, and other reagents BODIPY 493/503 Invitrogen D3922 Oleic Acid Sigma Aldrich 05508 Monodansylpentane (MDH) Abcepta SM1000a Water for UHPLC suitable for mass spectrometry (MS) Sigma Aldrich 900682-1L Acetonitrile, MS SupraSolv Sigma Aldrich 1006651000 Methanol, HPLC grade Sigma Aldrich 34860-1L-R Chloroform Sigma Aldrich C2432-1L Isopropanol Optima LC/MS Grade Fisher Scientific 10091304 Ammonium formate Sigma Aldrich 516961-100G SPLASH LpidoMIX Mass Spec Standard Avanti Polar Lipids 330707 Software Image J https://imagej.net/ij/ ImageJ 2.1.0/1.53c © The Author(s) The EMBO Journal 13 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number Imaris https:// imaris.oxinst.com/ Imaris 10.1.0 Prism https:// www.graphpad.com/ Prism 10.2.3 Adobe Photoshop https:// www.adobe.com/ Photoshop 2024 Other
High Performance Liquid Chromatography:Article Title: Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Saccharomyces cerevisiae Open Biosystems BY4741 and derivatives—Appendix Table S3 Recombinant DNA DNA plasmids Appendix Table S1 Appendix Table S1 Antibodies HRP Goat Anti-Rabbit IgG BD Biosciences 554021 Anti-Dgk1 Han et al, 2008b Han et al, 2008b Oligonucleotides and other sequence-based reagents Oligonucleotides Appendix Table S2 Appendix Table S2 Chemicals, enzymes, and other reagents BODIPY 493/503 Invitrogen D3922 Oleic Acid Sigma Aldrich 05508 Monodansylpentane (MDH) Abcepta SM1000a Water for UHPLC suitable for mass spectrometry (MS) Sigma Aldrich 900682-1L Acetonitrile, MS SupraSolv Sigma Aldrich 1006651000 Methanol, HPLC grade Sigma Aldrich 34860-1L-R Chloroform Sigma Aldrich C2432-1L Isopropanol Optima LC/MS Grade Fisher Scientific 10091304 Ammonium formate Sigma Aldrich 516961-100G SPLASH LpidoMIX Mass Spec Standard Avanti Polar Lipids 330707 Software Image J https://imagej.net/ij/ ImageJ 2.1.0/1.53c © The Author(s) The EMBO Journal 13 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number Imaris https:// imaris.oxinst.com/ Imaris 10.1.0 Prism https:// www.graphpad.com/ Prism 10.2.3 Adobe Photoshop https:// www.adobe.com/ Photoshop 2024 Other
Software:Article Title: Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models Saccharomyces cerevisiae Open Biosystems BY4741 and derivatives—Appendix Table S3 Recombinant DNA DNA plasmids Appendix Table S1 Appendix Table S1 Antibodies HRP Goat Anti-Rabbit IgG BD Biosciences 554021 Anti-Dgk1 Han et al, 2008b Han et al, 2008b Oligonucleotides and other sequence-based reagents Oligonucleotides Appendix Table S2 Appendix Table S2 Chemicals, enzymes, and other reagents BODIPY 493/503 Invitrogen D3922 Oleic Acid Sigma Aldrich 05508 Monodansylpentane (MDH) Abcepta SM1000a Water for UHPLC suitable for mass spectrometry (MS) Sigma Aldrich 900682-1L Acetonitrile, MS SupraSolv Sigma Aldrich 1006651000 Methanol, HPLC grade Sigma Aldrich 34860-1L-R Chloroform Sigma Aldrich C2432-1L Isopropanol Optima LC/MS Grade Fisher Scientific 10091304 Ammonium formate Sigma Aldrich 516961-100G SPLASH LpidoMIX Mass Spec Standard Avanti Polar Lipids 330707 Software Image J https://imagej.net/ij/ ImageJ 2.1.0/1.53c © The Author(s) The EMBO Journal 13 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number Imaris https:// imaris.oxinst.com/ Imaris 10.1.0 Prism https:// www.graphpad.com/ Prism 10.2.3 Adobe Photoshop https:// www.adobe.com/ Photoshop 2024 Other
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